-Ecopa Science Initiative. eSI-WORKSHOP. Alicante . Spain , 28-30/10/ 2004:

• R. Jover: Novel technologies and techniques: “Transfection with key transcription factors as a mean to metabolically upgrade human hepatic cells”.

• T. Vanhaecke and P. Papeleu: Use of chromatin remodelling as a new way to create differentiated primary hepatocyte cultures.

- Hepatocyte User Group Meeting. Valencia , Spain , 12-13/11/ 2004:

• R. Jover: Hepatocyte differentiation and gene expression. “Liver-enriched transcription factors and their role in preserving the hepatic phenotype in liver cell models”.

• M.T. Donato, A. Serralta, N. Jiménez, G. Pérez, J.V. Castell, J. Mir and M.J. Gómez-Lechón: Liver grafts preserved in Celsior solution as source of hepatocytes for drug metabolism studies: Comparison with surgical liver biopsies.

• C.P. Martímez-Jiménez, M.J. Gómez-Lechón, J.V. Castell and R. Jover: Transcriptional regulation of the human hepatic CY3A4 by the inflammatory signal interleukine-6: Molecular mechanism and transcription factors involved.

• T. Vanhaecke: Effect of HDAC inhibition on proliferation- and apoptosis-related signalling in EGF-stimulated primary rat hepatocyte cultures.

• T. Vanhaecke, P. Papeleu and V. Rogie Effect of HDAC inhibition on proliferation- and apoptosis-related signaling in EGF-stimulated primary rat hepatocyte cultures.

• M. Vinken, T. Vanhaecke, J.K. Chipman and V. Rogiers: The effect of Trichostatin A on gap junctional intercellular communication in primary cultures of rat hepatocytes.

- European ISSX Meeting at the Pharmaceutical Sciences Fair. Nice, France , 12-17/6/2005:

• J.V. Castell, R. Jover and M.J. Gómez-Lechón: Immortalised hepatocytes and drug metabolism: Strategies to overcome their constrains.

- 5 th World Congress on Alternatives and Animal Use in the Life Sciences, Berlin , Germany , 21-25/8/2005:

• J.V. Castell, A. Lahoz, D. Henández, M.J. Gómez-Lechón and R. Jover: A novel strategy applied on hepatocytes allowing to mimic in vitro the human metabolic idiosyncrasy.

• R. Jover , C.P. Martínez-Jiménez, M.J. Gómez-Lechón, J.V. Castell: Engineering human hepatoma cells with key transcription factors as a mean to generate metabolically competent human hepatic cell models .

• Jennings P, Aydin S, Koppelstaetter K, Mayer G, Pfaller W. : Cytomic assays for in vitro toxicity testing.

• H. J. Ahr, H. Ellinger-Ziegelbauer, G. Wasinska-Kempka, C. Weiland : Characterization of in vitro cultures of primary cells by expression profiling.

• T. Vanhaecke: Use of chromatin remodelling as a new way to create differentiated primary hepatocyte cultures.

• M. Vinken: The effect of Trichostatin A on gap junctional intercellular communication in primary cultures of adult rat hepatocytes.
• Cédric Duret, Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu and Patrick Maurel. Differenciation of hepatocyte-like cells from human embryonic stem cells and adult human liver progenitors.

- 46th ETCS International Meeting, Verona (2006)

• R Taléns-Visconti, A Bonora, R Jover, V Mirabet, F Carbonell, J.V. Castell and MJ Gomez-Lechon. Differentiatio of human mesenchymal stem cells from adipose tissue and bone marrow into hepatic lineage.

•  M.T. Donato, N. Jimenez, A. Serralta, J. Mir, J.V. Castell, M.J. Gómez-Lechón Effects of steatosis on drug-metabolising capability of primary human hepatocytes..

•  B. Cvek, J. Vrba, M. J. Gómez-Lechón y J. Ulrichova In vitro models in the prediction of hepatotoxicity in man.

- IX Congreso Nacional de la Asociación Española de Bancos de Tejidod (AEBT), Oviedo (2006)

• R Taléns-Visconti, A Bonora, R Jover, V Mirabet, F Carbonell-Uberos, JV Castell y MJ Gómez-Lechón. Diferenciación de células madre de tejido adiposo y médula ósea en células de linaje hepático.

- INVITOX, Ostende, (2006)

• M.J. Gómez-Lechón M.T. Donato, N. Jiménez and J.V. Castell. Impact of hepatocyte steatosis on hepatotoxicity of pharmaceutical drugs.

- ERCSG 18th Meeting Dublin 2006

• Paul Jennings , Christian Koppelstaetter, Sonia Aydin, Thomas Abberger, Anna-Maria Wolf, Gert Mayer and Walter Pfaller: Cyclosporine A induces senescence in cultured human proximal tubular epithelial cells via activation of the p53 pathway.
• C. Weiland: Characterization of a renal cell model by gene expression analysis

- American Society of Nephrology (ASN), Meeting Nov. 2005, Philadelphia

• Christian Koppelstaetter, Paul Jennings , Anna M. Wolf, Walter Pfaller: Cyclosporine A induces aging in renal tubular epithelial cells, JASN: 16, 33A (2005)
• Lorna Davey, Tara McMorrow, Michael P. Ryan: Differential effects of immunosuppressive agents in human renal proximal tubular cells, JASN: 16, 660A (2005)
• Craig Slattery, Tara McMorrow, Michael P. Ryan: The role of PKC- b in cyclosporine A –induced tubulointerstitial fibrosis, JASN: 16, 666A (2005)

- European Dialysis and Transplantation Association (EDTA), July 2006, Glasgow , Scotland .

• Christian Koppelstaetter, Paul Jennings , Sonia Aydin, Maria Wolf, Walter Pfaller and Gert Mayer: Cyclosporine A induces aging in renal epithelial cells (Award: 11 best abstracts presented by young authors)

- American Transplantation Society (ATS), Meeting, July 2006, Boston , USA .

• Christian Koppelstaetter, Paul Jennings , Sonia Aydin, Maria Wolf, Walter Pfaller and Gert Mayer: Cyclosporine A induces aging in renal epithelial cells

- 13th Congr on Alternatives to Animal Testing, Linz Austria , June 2006

• Thomas Lechleitner, Paul Jennings and Walter Pfaller: Trans-Epithelial Electrical Resistance monitoring in perfusion culture is a reliable indicator of monolayer integrity and barrier function .
• W. Pfaller: EpiFlow perfusion culture improves in vitro assessment of acute and chronic toxicit y.13th Congr on Alternatives to Animal Testing, Linz Austria, June 2006, Altex: 23 (2), 118 (2006)

- Tuesday Seminars in Molecular Medicine. Kirchberg. Luxembourg , 4/10/2005

• Vanhaecke T.: Use of chromatin remodelling as a new way to create differentiated primary hepatocyte cultures.

- Winterschool Nierstichting Nederland, Zandvoort, Netherlands, 11 januari 2006

• M.R. Daha. Common pathways of renal dysfunction; immunological aspects

  - Nederlandse Nefrologiedagen, Velthoven, Netherlands, 4-5 april 2006

• M.R. Daha. The inflamed kidney as a lymphoid organ

- Hopital Broussais, Paris, France 4 july 2006

• M.R. Daha . Production of cytokines and complement by tubular cells

- Mexican Immunology Society, Chihuhua, Mexico, 4 may 2006

• M.R. Daha . Is there a role for locally produced innate immune factors in renal inflammation?

- African Federation of Immunology Societies, Dakar , Senegal , 22 march 2006

• M.R. Daha . Inflammation in the kidney: role of immune factors and ischemia

- Conway Institute Festival of Research, Dublin , September 2006

• Geoff Watson, Gavin Ryan, Niamh Tuite and Michael P. Ryan: Analysis of the transcriptional response to immunosuppressive agents in human renal epithelial cells: insight into genes involved in epithelial-mesenchymal transition

- European Renal Cell Study Group, Dublin , May 2006

• Sein O'Connell, Michael P. Ryan: Generation of an in vivo model of cyclosporine A-induced renal fibrosis.

- 45th Annual Meeting of the Society of Toxicology, San Diego 2006

• A. Wolf, mitochondrial f1-atp synthase: no functional consequences of rna interferencemediated gene silencing in hepg2

Annual Ecopa workshop in December 2005 in Brussels

• B. Garthoff. Thereby laid out the concept to a broad audience of stakeholders, i. e. Academia, Animal Welfare, Governmental Institutions incl. EU Commission officials, and Industries as well as Trade Associations.

Congreso de la Sociedad Española de Biología Celular (SEBC). Pamplona (2007).

• Nuevas alternativas al transplante de órgano entero: Diferenciación de células madre mesenquimales adultas hacia linaje hepático A Bonora-Centelles, Jover R, R. Talens-Visconti, Castell JV and Gómez-Lechón MJ.

Congreso de la Sociedad Ibérica de Citometría. Barcelona (2007)

• Application of high-content analysis to explore functional endpoints of cytotoxicity inprimary cultures of hepatocytes. Sandra Pinto, Laura Diaz, Guadalupe Herrera, Maria-José Gomez-Lechón, Teresa Donato, José-Vicente Castell, Robert C. Callaghan and José-Enrique O’Connor.

High-Content Analysis Workshop, Madrid, Spain, 26-27 March 2007.

•  Pinto, S.,  Diaz, L.,  Herrera, G., Gomez-Lechón, M.J.,  Donato, T., Castell, J.V.,  Callaghan, R.C., O’Connor, J E. Application of High-Content Analysis to Explore Functional Endpoints of Citotoxicity in Primary Cultures of Hepatocytes.

European Meeting of the Society for Free Radical Research, Vilamoura, Portugal, 10-13 October 2007. (2007)

•  'Abstracts', Free Radical Research, 41:1, 23 (P-137). Pinto, S., Diaz, L., Herrera, G., Gómez-Lechón, M.J., Donato, T., Castell, J.V., O´Connor, J.E. High Content Bioimaging Assays for Oxidative Stress Assessment in Primary Cultures of Hepatocytes.

Congreso de la Sociedad Ibérica de Citometría. Barcelona (2007).

• The role of cytomics to predict long-term hepatotoxicity: in vitro short-term assay for steatosis and cholestasis  Martínez-Romero, Alicia; Díaz, Laura;   Pinto, Sandra; Gómez-Lechón, M.José; Rohacova, Jana; Marín, Maria-Luisa; Miranda, Miguel Angel; Castell, José-Vicente and O´Connor, José- Enrique.

Congreso de la Sociedad Ibérica de Citometría. Barcelona (2007).

•  High-content analysis in vitro to predict long-term toxicity in the liver. Pinto, Sandra; Gómez-Lechón, María-José; Martínez-Romero, Alicia; Díaz, Laura; Castell, José-Vicente and O´Connor, José-Enrique.
• High-Content Analysis Workshop, Madrid, Spain, 26-27 March 2007. Pinto, S.,  Diaz, L.,  Herrera, G., Gomez-Lechón, M.J.,  Donato, T., Castell, J.V.,  Callaghan, R.C., O’Connor, J E. Application of High-Content Analysis to Explore Functional Endpoints of Citotoxicity in Primary Cultures of Hepatocytes.

     San Francisco J Am Soc Nephrol 18 October 2007 P138A.

• Aydin S, Signorelli S, Lechleitner T, Joannidis M, Pfaller W and Jennings P, Investigation of the Role of the Microvascular Endothelium in Transcriptional Regulation of Proximal Tubular Epithelial Cells Using an In Vitro Co-Culture System.

• Koppelstaetter C, Kern G, Jennings P, Schaumeier M, Pfaller W, Mayer G. Signs of Cellular Senescence in Cyclosporine A and Tacrolimus Treated Renal Tubule Cells.

·     14 th Congress on Alternatives to Animal Testing. Linz Austria Sept. 2007. ALTEX: 24 (03), P202.

• Jennings P. Standardisation of cell culture techniques: a prerequisite to effective multi laboratory comparison of microarray data.  Oral presentation.

at INVITOX 2006 14th International Workshop on in Vitro Toxicology; from 02/10/2006 to 05/10/2006,

•  Ostend, Belgium: Henkens T., Papeleu P., Elaut G., Vinken M., Rogiers V. and Vanhaecke T.: ‘Trichostatin A, a critical factor to maintain differentiation in primary cultures of rat hepatocytes.’

at the FP7 Environmental Technologies Info Day 23/01/2007, Brussels, Belgium:

•   Vanhaecke T.: ‘VUB Dept. Toxicology : Research competence.’

at the Journée exceptionnelle CONNEXINES ; 25/05/2007, Paris, France :

•  Vinken M., Henkens T., Vanhaecke T., Geerts A., Chipman J.K., Meda P. and Rogiers V. : ‘Les effets de la trichostatine A, un inhibiteur d’histone désacétylase, sur l’expression des connexines dans des cultures primaires d’hépatocytes du rat: résultats et perspectives.’

at the ICT Meeting; 16/07/2007, Montréal, Canada:

• Chipman J.K., Vinken M., Edwards G., Vanhaecke T. and Rogiers V.: ‘The role of connexin 32 in liver cell proliferation and differentiation.’

at the 6^th World Congress on Alternatives and Animal Use in the Life Sciences; from 21/08/2007 to 25/8/2007 , Tokyo, Japan:

• Rogiers V.: ‘Histone Deacetylase Inhibitors (HDACi): Opening a New Field of Research in the Development of Alternative Methods ?’

at the Dertiende Forum der Farmaceutische Wetenschappen (BGFW); from 11/10/2007 to 12/10/2007, Spa, Belgium:

• Fraczek J., Deleu S., Vanhaecke T., Vanderkerken K. and Rogiers V.: ‘Growth inhibitory and differentiating properties of novel hydroxamate-based Histone Deacetylase Inhibitors in cultures of primary rat hepatocytes.’
• at the Dertiende Forum der Farmaceutische Wetenschappen (BGFW); from 11/10/2007 to 12/10/2007, Spa, Belgium: De Rop E., Papeleu P., Vanhaecke T. and Rogiers V.: ‘Synergetic effects of histon acetylation and DNA demethylation on proliferation and differentiation in EGF-stimulated primary rat hepatocytes.’

at the Knowledge for Growth Annual Flemisch Biotech Convention, 8/6/2007, Ghent, Belgium:

• Vinken M., Henkens T., Vanhaecke T., Geerts A., Chipman J.K., Meda P. and Rogiers V.: ‘Trichostatin A enhances gap junctional intercellular communication in primary cultures of adult rat hepatocytes.’

at the Discovery on Target 2007: histone deacetyalse inhibitors, 16/10/2007-17/10/2007, Boston: USA:

• Vinken M., Henkens T., Vanhaecke T., Geerts A., Chipman J.K., Meda P. and Rogiers V.: ‘Trichostatin A enhances gap junctional intercellular communication in primary cultures of adult rat hepatocytes.’

16th International Symposium on Microsomes and Drug Oxidation (MDO 2006). Budapest Hungaria, 3-7 September 2006.

• P Maurel. Chairman of Symposium on Regulation of Gene Expression.

15th International Conference on Cytochromes P450. Session:

• Transcriptional Regulation. Bled Slovenia, 17-21 June 2007. Patrick Maurel: Introduction: Transcription factors, nuclear receptors, and coreceptors involved in the control of drug metabolism.

Symposium in Honor of Dr UA Meyer. Biozentrum, University of Basel. Basel Switzerland, 22 June 2007.

• J.M. Pascussi, A. Moreau, A. Robert, S. Gerbal-Chaloin, C. Duret, M. Daujat-Chavanieu, M.J. Vilarem, P. Maurel. Nuclear receptors involved in the control of drug metabolism: crosstalk and consequences.

11th International Congress of Toxicology. Montreal Canada, 15-19 July 2007.

• J.M. Pascussi, A. Moreau, A. Robert, S. Gerbal-Chaloin, C. Duret, M.Daujat-Chavanieu, M.J. Vilarem, P Maurel. Nuclear receptors involved in the control of drug metabolism: crosstak and consequences.

8th International ISSX Meeting. Sendai Japan, 9-12 October 2007.

• J.M. Pascussi, A. Moreau, A. Robert, S. Gerbal-Chaloin, C. Duret, M.. Daujat-Chavanieu, M.J. Vilarem, P. Maurel. Nuclear receptors: crosstalk and consequences.

17th International Symposium on Microsomes and Drug Oxidations, Saratoga Springs NY USA July 6-10 2008.

• Patrick Maurel: Chairman of Symposium 8: Stem cell-based new cellular systems for drug metabolism and toxicity testing.

17th International Symposium on Microsomes and Drug Oxidations, Saratoga Springs NY USA July 6-10 2008.

• Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu, Natalie Funakoshi, Cédric Duret, Astrid Herrero, and  Patrick Maurel. Generation of hepatocytes from human adult and embryonic stem cells.

Winterschool Duth Kidney Foundation, Zandvoort, 11 January 2007.

• Use of isolated kidney cells to assess nephrotoxic function

International congress of Immunology, Rio de Janeiro, Brazil, 23 August 2007.

• Immunological consequence of nephrotoxins on isolated renal cells

Bantao congress, Belgrade, Serbia, 17 September 2007.

• Assessment of nephrotoxins using isolated renal cells

Hopital Broussais, Paris, France 4 July 2006

• Production of cytokines and complement by tubular cells

American Society of Nephrology Conference. San Francisco, USA.

• Feighery, R., Ryan, M.P. and McMorrow, T. (2007) Proteomic Approaches to Immune-Mediated Epithelial Mesenchymal Transition (EMT) in Renal Disease.

7th Annual ECOPA Workshop 2006, Brussels. Oral presentation.

• Weiland: Characterization of a renal cell model by gene expression analysis.

Annual ecopa workshop in November 2006 and November 2007 in Brussels,

•  and thereby laid out the concept to a broad audience of stakeholders, i. e. Academia, Animal Welfare, Governmental Institutions incl. EU Commission officials, and Industries as well as Trade Associations

• (Up)

• •  Martínez-Jiménez C.P., Jover R., Gomez-Lechon M.J. and J.V. Castell. Can hepatoma cell lines be re-differentiated to be used in drug metabolism studies? ATLA 32 (Supplement 1): 65-74, 2004

• •  M.T. Donato, A. Serralta, N. Jiménez, G. Pérez, J.V. Castell, J. Mir and M.J. Gómez-Lechón. Liver grafts preserved in Celsior solution as source of hepatocytes for drug metabolism studies: Comparison with surgical liver biopsies. Drug Metabolism and Disposition 33:1-7, 2005.

• •  C.P. Martínez-Jiménez, M.J. Gomez-Lechon, J.V. Castell and R. Jover. Transcriptional regulation of the human hepatic CYP3A4: Identification of a new distal enhancer region responsive to CCAAT/enhancer binding protein beta isoforms (LAP and LIP). Molecular Pharmacology 67(6):2088-2101, 2005.

• •  E. O´Connor, A. Martínez, J. V. Castell and M. J. Gómez-Lechón. Multiparametric characterization by flow cytometry of flow-sorted subpopulations of a human hepatoma cell line useful for drug research.. Cytometry A. 63,48-58 (2004)

• •  Martinez-Jimenez CP, Gomez-Lechon MJ, Castell JV, Jover R. Under-expressed coactivators PGC1alpha and SRC1 impair HNF4alpha function and promote dedifferentiation in human hepatoma cells. J Biol Chem. 2006 Aug 4; [Epub ahead of print]

• •  Martinez-Jimenez CP, Castell JV, Gomez-Lechon MJ, Jover R. Transcriptional activation of CYP2C9, CYP1A1 and CYP1A2 by HNF4{alpha} requires coactivators PGC1{alpha} and SRC1. Mol Pharmacol. 2006 Aug 1; [Epub ahead of print]

• •  Castell JV, Jover R, Martinez-Jimenez CP, Gomez-Lechon MJ. Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies. Expert Opin Drug Metab Toxicol. 2006 Apr;2(2):183-212.

• •  Martínez-Jiménez C.P., Gomez-Lechon M.J., Castell J.V., Jover R. Engineering human hepatoma cells with key transcription factors to generate metabolically competent hepatic models. ALTEX 22 (Special Issue 2), 387-392. 2005

• •  A. Serralta, M.T. Donato, A. Martinez, E. Pareja, F. Orbis, J. V. Castell, J. Mir y M. J. Gómez-Lechón. Influence of preservation solution on the isolation and culture of human hepatocytes from liver grafts. Cell Transplant 14:837-843 (2005).

• •  M. J. Gómez-Lechón, A. Lahoz, Nuria Jimenez, J.V. Castell, M.T. Donato. Cryopreservation of hepatocytes: Influence of preculture and cryoprotectants on recovery, cytochrome P450 activities and induction upon thawing. Xenobiotica 36:457-472. (2006).

• •  M. T. Donato and M. J. Gómez-Lechón. Inhibition of P450 enzymes: An in vitro approach to predict drug-drug interactions. Current Enzyme Inhibition 2:281-304 (2006).

• •  M. Teresa Donato, Agustín Lahoz, Nuria Jiménez, Gabriela Pérez, Alfonso Serralta, José Mir, José V. Castell, and M. J. Gómez-Lechón. Effects of steatosis on P450 enzymes: Potential impact on clinical and experimental applications of human hepatocytes isolated from fat liver grafts. Drug Metab. Disp. 34:1556-1562 (2006)

• •  Th. Abberger, P. Jennings, A. Mirlach, S. Prajczer and W. Pfaller Application of a population balance model to a perfusion in vitro toxicity system. Toxicol. In Vitro: 20(7):1213-24 (2006)

• •  Bijuklic, K., Sturn, D.H., Jennings, P., Kountchev, J., Pfaller, W., Wiedermann, C.J., Patsch, J.R., and Joannidis, M. (2006). Mechanisms of Neutrophil Transmigration Across Renal Proximal Tubular HK-2 Cells. Cell Physiol Biochem 17, 233-244.

• •  Koppelstaetter, C., Jennings, P., Hochegger, K., Perco, P., Ischia, R., Karkoszka, H., and Mayer, G. (2005). Effect of tissue fixatives on telomere length determination by quantitative PCR. Mechanisms of ageing and development 126, 1331-1333

• •  Elaut G., Vanhaecke T., Vander Heyden Y. and Rogiers V. (2005) Spontaneous apoptosis, necrosis, energy status, glutathione levels and biotransformation capacities of isolated rat hepatocytes in suspension: effect of the incubation medium, Biochemical Pharmacology 69, 1829-1838.

• •  Papeleu P., Vanhaecke T., Elaut G., Vinken M., Henkens T., Snykers S. and Rogiers V. (2005) Differential effects of histone deacetylase inhibitors in tumor and normal cells. What is the toxicological relevance ?, Critical Reviews in Toxicology 35, 363-378.

• •  Vanhaecke T., Vinken M., Henkens T., Snykers S., Elaut G., Papeleu P. and Rogiers V. (2005) Effects of Trichostatin A on apoptosis-regulating proteins during hepatocyte isolation, ALTEX 23, 441-444.

• •  Vinken M., Vanhaecke T., Papeleu P., Snykers S., Henkens T., Chipman J.K. and Rogiers V. (2005) A study of connexin expression in monolayer cultures of primary rat hepatocytes, ALTEX 23, 456-460.

• •  V. Rogiers and M. Pauwels. (2005) Good science must be the key factor in the development and use of alternative methods for safety assessment of cosmetics, ALTEX 22, 259.

• •  Vinken M., Vanhaecke T., Papeleu P., Snykers S., Henkens T. and Rogiers V. (2006) Connexins and their channels in cell growth and cell death, Cellular Signalling 18, 592-600.

• •  Papeleu P., Vanhaecke T. and Rogiers V. (2006) Histone deacetylase inhibition: a differentiation therapy for cultured primary hepatocytes ?, Current Enzyme Inhibition 2, 91-104.

• •  Vinken M., Henkens T., Vanhaecke T., Papeleu P., Geerts A., Van Rossen E., Chipman J.K., Meda P. and Rogiers V. (2006) Trichostatin A enhances gap junctional intercellular communication in primary cultures of adult rat hepatocytes, Toxicological Sciences 91 (2), 484-492.

• •  Vinken M., Papeleu P., Snykers S., De Rop E., Henkens T., Chipman J.K., Rogiers V. and Vanhaecke T. (2006) Involvement of cell junctions in hepatocyte culture functionality, Critical Reviews in Toxicology 36, 299-318.

• •  Vinken M., Papeleu P., Rogiers V. and Vanhaecke T. (2006) Histone deacetylase inhibitors as potent modulators of cellular contacts. Current Drug Targets 7, 773-787.

• •  Elaut G., Henkens T., Papeleu P., Snykers S., Vinken M., Vanhaecke T. and Rogiers V. (2006) Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures, Current Drug Metabolism7 (6), 629-660.

• •  Vanhaecke T. and Rogiers V. Hepatocyte cultures in drug metabolism and toxicological research and testing, In: Cytochrome P450 Protocols. Series in: Methods in Molecular Biology (Phillips I.R. and Shephard E.A., eds.), vol 320, pp. 209-227, Humana Press Inc., Totowa, NJ, 2006.

• •  Papeleu P., Vanhaecke T., Henkens T., Elaut G., Vinken M., Snykers S. and Rogiers V. Isolation of rat hepatocytes, In: Cytochrome P450 Protocols. Series in: Methods in Molecular Biology (Phillips I.R. and Shephard E.A., eds.), vol 320, pp. 229-237, Humana Press Inc., Totowa, NJ, 2006.

• •  Vinken M., Elaut G., Henkens T., Papeleu P., Snykers S., Vanhaecke T. and Rogiers V. Rat hepatocyte cultures: Collagen gel sandwich and immobilization cultures, In: Cytochrome P450 Protocols. Series in: Methods in Molecular Biology (Phillips I.R. and Shephard E.A., eds.), vol 320, pp. 255-263, Humana Press Inc., Totowa, NJ, 2006.

• •  De Haij, S., A.M. Woltman, L.A. Trouw, A.C. Bakker, S.W. Kamerling, S.W. van der Kooij, L. Chen, R.A. Krozcek, M.R. Daha and C. van Kooten. 2005. Renal tubular epithelial cells modulate T cell responses via ICOS-L and B7-H1. Kidney Int. 68:2091-2102

• •  Slattery, C., Campbell, E., McMorrow, T. & Ryan, M.P: Cyclosporine A-induced renal fibrosis: a role for epithelial-mesenchymal transition.
  Am J Pathol. 2005 Aug;167(2):395-407.

• •  McMorrow, T., Gaffney, M.M., Slattery, C., Campbell, E. & Ryan, MP: Cyclosporine A induced epithelial-mesenchymal transition in human renal proximal tubular epithelial cells.
  Nephrol Dial Transplant. 2005 Oct;20(10):2215-25.

• •  Slattery, C., McMorrow, T. & Ryan, M.P Overexpression of E2A proteins induces epithelial-mesenchymal transition in human renal proximal tubular epithelial cells suggesting a potential role in renal fibrosis.
  FEBS Lett. 2006 Jul 24;580(17):4021-30.

• •  C. Strupp, F. Straube, O. Grenet, W. E. Trommer and A. Wolf . mitochondrial f1-atp synthase: no functional consequences of rna interference-mediated gene silencing in hepg2, Toxicologist, 1716, 351-352,

• •  Garthoff. B. EU 6th Framework Programme projects"/ PREDICTOMICS. "messenger" no 5, 6, 7 and 8 ( i.e. 5: Nov. 2005; 6: Dec. 2005, 7: March 2006; 8: July 2006).

• Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue: Compasrison with bone marrow mesenchymal stem cells. R. Talens-Visconti, A. Bonora-Centelles, R. Jover, V.Mirabet, F. Carbonell, J. V. Castell, M. J. Gómez-Lechón. World J. Gastroenterol  28: 5834-5845 (2006).

• Potential impact of steatosis on cytochrome P450 enzymes of human hepatocytes isolated from fatty liver grafts. Donato MT, Lahoz A, Jimenez N, Perez G, Serralta A, Mir J, Castell JV, Gomez-Lechon MJ. Drug Metab Dispos. 34:1556-1562. 2006.

• Engineering human hepatoma cells with key transcription factors to generatemetabolically competent hepatic models. Martinez-Jimenez CP, Gomez-Lechon MJ, Castell JV, Jover R.  ALTEX 23:4 35-440. 2006

• Cytomics: A multiparametric, dynamic approach to cell research. Herrera, G., Diaz, L., Martinez-Romero, A., Gomes, A., Villamón, E., Callaghan, R.C., O'Connor, J.E. (2007) Toxicol. In Vitro. 21:176-182.

• A human hepatocellular in vitro model to investigate steatosis. M.J.  Gómez-Lechón, M. T. Donato, A. Martínez-Romero,  N. Jiménez, J. V. Castell  y  E. O´Connor. Chemico-Biol Interact 165:106-116 (2007).

• Effects of steatosis on drug-metabolizing capability of primary human hepatocytes. M. T. Donato, N. Jiménez, A. Serralta, J. Mir, J. V Castell y M. J. Gómez-Lechón. Toxicol In vitro 21: 271-276 (2007).

• Human mesenchymal stem cells from adipose tissue: Differentiation into hepatic lineage. R Taléns-Visconti, A Bonora, R Jover, V Mirabet, F Carbonell, J.V. Castell, MJ Gomez-Lechon. Toxicol In vitro 21:330-334 (2007).

• CYP3A4 regulation and expression in hepatocytes C. P. Martínez-Jiménez, R. Jover, M. T. Donato, J. V. Castell and M. J. Gómez-Lechón. Curr Drug Metab. 8:185-195 (2007)

• Primary hepatocytes: Current understanding of the regulation of metabolic enzymes, transporter proteins and how they are used in pharmaceutical practice. N. J. Hewitt, M. J. Gómez Lechón, J. B. Houston, D. Hallifax, H. S. Brown, P. Maurel, B. McGee, S. Lloyd, P.ip G. Hewitt and J. G. Hengstler. Drug Metab. Rev 39:159-234 (2007).

• In vitro screening of drug metabolism in drug development.  Gómez-Lechón  M.J., Lahoz A.  Y   Donato. M.T. SCREENING  Trends in Drug Discovery 8:32-33 (2007)

• In vitro investigation of drug metabolism and toxicity in man. M. J. Gómez-Lechón and  M. T. Donato. Anales de la real academia Nacional de Farmacia 73:5-26 (2007).

• Hepatocytes the choice to investigate drug metabolism and toxicity in man: In vitro variability as a reflection of in vivo. M. J.  Gómez-Lechón, J. V. Castell and M. T. Donato. Chemico-Biol Interact 168:30-50 (2007).

• Determination of major human cytochrome P450s activities in 96-well plates using liquid chromatography tandem mass spectrometry. A. Lahoz, M.T. Donato, L. Picazo, M.J. Gómez-Lechón and J.V. Castell. Toxicol In vitro 21:1247–1252 (2007).

• Strategies to in vitro assessment of major human CYP enzyme activities by using liquid chromatography tandem mass spectrometry. A. Lahoz, M. T. Donato, J. V Castell and M. J. Gómez-Lechón. Curr Drug Metab. (in press)

• Cell lines: A tool for in vitro drug metabolism studies. Gómez-Lechón M. J., Lahoz A., Castell J. V. Y Donato M. T. Curr Drug Metab. (in press)

• ATF5, a highly abundant liver-specific transcription factor, cooperates with CAR in the transactivation of CYP2B6: Implications in hepatic stress response. M. Pascual, M. J. Gómez-Lechón, J.V. Castell y R. Jover. Drug Metab Disp (in press)

• Evaluation of drug-metabolising and functional competence of human hepatocytes incubated under hypothermia in different media for clinical infusion. Gómez-Lechón M. J., Lahoz A., Jiménez N., Bonora A.,  Castell J. V. and Donato M.T. Cell transplant (in press)

• In vitro P450 inhibition/induction in early drug Development. M. Teresa Donato, José V. Castell and M. José Gómez-Lechón Expert Opinion on Drug Metabolism and Toxicology (in press)

• Novel flow cytometric kinetic in vitro assays to predict long-term effects of cholestatic xenobiotics. Gómez-Lechón, M.J., Díaz, L., Donato, M.T., Martínez-Romero, A., Castell, J.V., O’Connor, J.E..Toxicological Sci. (Submitted)

• Cytomic features of a human cellular model suitable to investigate hepatic steatosis in vitro. Martínez-Romero, A., Gómez-Lechón, MJ, Díaz, L., Donato, M.T., Castell, J.V., O’Connor, J.E.  Cytometry part A (In Press, 2008)

• Cytomic in vitro assays to predict drug-induced liver steatosis Gómez-Lechón, M.J., Díaz, L., Donato, M.T., Martínez-Romero, A., Castell, J.V., O’Connor, J.E. Toxicological Sci. (Submitted)

• Photophysical characterization and cytomic applications of cholylamidofluorescein, a fluorescent bile acid scaffold. Jana Rohacova, M. Luisa Marin, Enrique O’Connor, M. Jose Gomez-Lechon, M. T. Donato, Jose V. Castell, Miguel A. Miranda..  Analytical Biochemistry (In press)

• Cytomics: A multiparametric, dynamic approach to cell research. Herrera, G., Diaz, L., Martinez-Romero, A., Gomes, A., Villamón, E., Callaghan, R.C., O'Connor, J.E. (2007) Toxicol. In Vitro. 21:176-182.

• Aydin S, Signorelli S, Lechleitner T, Joannidis M, Pleban C, Perco P, Pfaller W and Jennings P. The influence of microvascular endothelial cells on transcriptional regulation of proximal tubular epithelial cells. Am J Physiol Cell Physiol, 2007.

• Bijuklic K, Jennings P, Kountchev J, Hasslacher J, Aydin S, Sturn D, Pfaller W, Patsch JR, and Joannidis M. Migration of leukocytes across an endothelium-epithelium bilayer as a model of renal interstitial inflammation. Am J Physiol Cell Physiol, 2007.

• Jennings P, Koppelstaetter C, Aydin S, Abberger T, Wolf AM, Mayer G, and Pfaller W. Cyclosporine A induces senescence in renal tubular epithelial cells. Am J Physiol Renal Physiol, 2007.

• Lechner J, Malloth N, Seppi T, Beer B, Jennings P, and Pfaller W. IFN alpha induces barrier destabilization and apoptosis in renal proximal tubular epithelium. Am J Physiol Cell Physiol, 2007.

• Henkens T., Papeleu P., Elaut G., Vinken M., Rogiers V. and Vanhaecke T. (2007) Trichostatin A, a critical factor in maintaining the functional differentiation of primary cultures rat hepatocytes, Toxicology and Applied Pharmacology 218 (1), 64-71.

• Papeleu P., Wullaert A., Elaut G., Henkens T., Vinken M., Laus G., Tourwé D., Beyaert R., Rogiers V. and Vanhaecke T. (2007) Inhibition of NF-KB activation by the histone deacetylase inhibitor 4-Me₂N-BAVAH induces an early G1 cell cycle arrest in primary hepatocytes. Cell Proliferation 40 (5), 640-655. Corrigendum in Cell Proliferation 2007, 40, 96.

• Elaut G., Laus G., Alexandre E., Richert L., Tourwé D., Rogiers V. and Vanhaecke T. (2007) A metabolic screening study of Trichostatin A (TSA) and TSA-like histone deacetylase inhibitors in rat and human primary hepatocyte cultures. Journal of Pharmacology and Experimental Therapeutics 321 (1), 400-408.

• Vinken M., Henkens T., Snykers S., Lukaszuk A., Tourwé D., Rogiers V. and Vanhaecke T. (2007) The novel histone deacetylase inhibitor 4-Me₂N-BAVAH differentially affects cell junctions between primary hepatocytes, Toxicology 236 (1-2): 92-102.

• Elaut G., Rogiers V. and Vanhaecke T. (2007) The pharmaceutical potential of histone deacetylase inhibitors, Current Pharmaceutical Design 13, 2584-2620.

• Ketoconazole and miconazole are antagonists of the human glucocorticoid receptor: consequences on the expression and function of the constitutive androstane receptor and the pregnane X receptor. Duret C, Daujat-Chavanieu M, Pascussi JM, Pichard-Garcia L, Balaguer P, Fabre JM, Vilarem MJ, Maurel P, Gerbal-Chaloin S. Mol Pharmacol. 2006 Jul;70(1):329-39.

• Isolation, characterization and differentiation to hepatocyte-like cells of non parenchymal epithelial cells from adult human liver. Duret C, Gerbal-Chaloin S, Ramos J, Fabre J.M, Jacquet

• Nee, L., Tuite, N., Ryan M.P., McMorrow T. (2007) TNF-a and IL-1b-Mediated Regulation of MMP-9 and TIMP-1 in Human Glomerular Mesangial Cells. Nephron: Experimental Nephrology 107(2):73-86

• Slattery, C., Ryan, M.P., McMorrow, T. (2007) E2A proteins: Regulators of cell phenotype in normal physiology and disease. International Journal of Biochemistry & Cell Biology, doi:10.1016/j.biocel.2007.05.014

• Feldman, G., Kiely, B., Martin, N., Ryan, G., McMorrow, T. and Ryan, M.P. (2007) Role for TGF-Beta in cyclosporine-induced modulation of renal epithelial barrier function. Journal of the American Society of Nephrology, 18, (6):1662-1671.

• Weiland C., Ahr HJ., Vohr, HW., Ellinger-Ziegelbauer H. (2007) Characterization of primary rat proximal tubular cells by gene expression analysis. Toxicology In Vitro 21, 466-491

•  Ph.D. Thesis: Weiland, C. (2007) Mechanistische Analyse der Nephrokarzinogenese mit Hilfe von Expressionsanalysen in vivo und in vitro. Heinrich-Heine Universitaet, Duesseldorf

• The results, publishable executive summary and reports of the board meetings were presented to the EU stakeholder community in the ecopa newsletter under the heading "EU 6th Framework Programme projects"/ PREDICTOMICS. These were given in the editions of the "messenger" no 9, 10, 11, 12, 13 and X-mas edition ( i.e. 31/10/2006, 16/12/2006, 31/03/2007, 30/06/2007, 03/11/2007 and 24/12/2007).

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•  R. Talens-Visconti, A. Bonora-Centelles, R. Jover, V.Mirabet, F. Carbonell, J. V. Castell, M. J. Gómez-Lechón. Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue: Compasrison with bone marrow mesenchymal stem cells. World J. Gastroenterol (in press).

•  M. J. Gómez-Lechón, J. V. Castell and M. T. Donato. Hepatocytes the choice to investigate drug metabolism and toxicity in man: In vitro variability as a reflection of in vivo.. Chemico-Biol Interact. (in press).

•  T. Donato, A. Martínez-Romero, N. Jiménez, J. V. Castell y E. O´Connor. A human hepatocellular in vitro model to investigate steatosis. M.J. Gómez-Lechón, M. Chemico-Biol Interact. (in press).

•  M. T. Donato, N. Jiménez, A. Serralta, J. Mir, J. V Castell y M. J. Gómez-Lechón. Effects of steatosis on drug-metabolizing capability of primary human hepatocytes. Toxicol In vitro (in press).

•  C. P. Martínez-Jiménez, R. Jover, M. T. Donato, J. V. Castell2 and M. J. Gómez-Lechón CYP3A4 regulation and expression in hepatocytes. Curr. Drug Metab. (in press).

•  R Taléns-Visconti, A Bonora, R Jover, V Mirabet, F Carbonell, J.V. Castell, MJ Gomez-Lechon.Human mesenchymal stem cells from adipose tissue: Differentiation into hepatic lieage. Toxicol In vitro (in press)

•  Papeleu P., Wullaert A., Elaut G., Henkens T., Vinken M., Laus G., Tourwé D., Beyaert R., Rogiers V. and Vanhaecke T. (2006) Inhibition of NF- K B activation by the histone deacetylase inhibitor 4-Me2N-BAVAH induces an early G1 cell cycle arrest in primary hepatocytes, Cell Proliferation. (Up)

• M.T. Donato, E. O´Connor, A. Serralta, J.V. Castell, J. Mir and M. J. Gómez-Lechón. Potential impact of steatosis on human hepatocyte transplantation: Effects on drug-metabolising capacity and Fas-induced apoptosis, Cell Transplant , 2005.

• M.J. Gómez-Lechón, M.T. Donato, A. Serralta, J. Mir and J.V. Castell. Cryopreserved human hepatocytes from monolayer cultures as a source for cell transplantation and culture , Drug Metabolism and Disposition , 2005.

• R. Jover, C.P. Martínez-Jiménez, M.J. Gomez-Lechon and J.V. Castell. Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies, Expert Opinion on Drug Metabolism and Toxicology , 2005.

•  Elaut G., Laus G., Alexandre E., Vanhaecke T., Papeleu P., Richert L., Tourwé D. and Rogiers V. (2006) A metabolic screening study of Trichostatin A (TSA) and TSA-like histone deacetylase inhibitors in rat and human primary hepatocyte cultures, Journal of Pharmacology and Experimental Therapeutics.

•  Elaut G., Vanhaecke T., Hebbrecht S., De Rop E., Henkens T., Papeleu P., Snykers S., Vinken M. and Rogiers V. (2006) Toxicological screening of Trichostatin A and its synthetic analogue 4-Me2N-BAVAH in cultured rat hepatocytes. Toxicological Sciences.

•  Henkens T., Papeleu P., Elaut G., Vinken M., Rogiers V. and Vanhaecke T. (2006) Trichostatin A, a critical factor to maintain differentiation on primary cultures of rat hepatocytes, Toxicology and Applied Pharmacology.

•  Vinken M., Henkens T., Papeleu P., Rogiers V., Lukaszuk A., Tourwé D. and Vanhaecke T. (2006) Differential effects of hydroxamate histone deacetylase inhibitors on cell junctions between primary hepatocytes, Biochemical Pharmacology.

•  Elaut G., Rogiers V. and Vanhaecke T. (2006) The pharmaceutical potential of histone deacetylase inhibitors, Current Pharmaceutical Design.

•  Elaut G., Jondhale S., Vanhaecke T., Tourwé D., Laus G., Chipman J.K. and Rogiers V. (2006) Predictive power of Meteor and Derek for Windows: a case study with the histone deacetylase inhibitors Trichostatin A and 4-Me2N-BAVAH, Archives of Toxicology.

• Cédric Duret, Sabine Gerbal-Chaloin, Jeanne Ramos, Jean-Michel Fabre, Eric Jacquet, Francis Navarro, Pierre Blanc, Antonio Sa-Cunha, Patrick Maurel and Martine Daujat-Chavanieu ‘Isolation, characterization and differentiation to hepatocyte-like cells of non parenchymal epithelial cells from normal adult human liver” (submitted).

• Vinken M., Henkens T., De Rop E., Fraczek J., Vanhaecke T. and Rogiers V. (2007) Biology and pathobiology of gap junctional channels in hepatocytes. Hepatology.

• Henkens T., Vinken M., Vanhaecke T. and Rogiers V. (2007) Modulation of CYP1A1 and CYP2B1 expression upon cell cycle progression in cultures of primary rat hepatocytes, Toxicology in Vitro.

• Snykers S., Vinken M., Henkens T., De Rop E., Fraczek J., Elaut G., Rogiers V. and Vanhaecke T. (2007) Epigenetic alterations in in vitro toxicology: potential for functional long-term cultures of primary and progenitor-derived hepatocytes, Expert Opinion on Drug Metabolism & Toxicology.

• Elaut G., Vanhaecke T., Hebbrecht S., De Rop E., Henkens T., Papeleu P., Snykers S., Vinken M. and Rogiers V. (2007) Toxicological screening of Trichostatin A and its synthetic analogue 4-Me2N-BAVAH in cultured rat hepatocytes. Toxicological Sciences.

• Elaut G., Jondhale S., Vanhaecke T., Tourwé D., Laus G., Chipman J.K. and Rogiers V. (2007) Predictive power of Meteor and Derek for Windows: a case study with the histone deacetylase inhibitors Trichostatin A and 4-Me2N-BAVAH, Archives of Toxicology.

• Henkens T., Vinken M., Lukaszuk A., Tourwé D., Rogiers V. and Vanhaecke T. (2007) Differential effects of hydroxamate histone deacetylase inhibitors on cellular functionality and gap junctions in primary cultures of mitogen-stimulated hepatocytes. Toxicology Letters

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• G . Elaut, J.K. Chipman and V. Rogiers . Predictive power of METEOR/DEREK: a case study with Trichostatin A and 4-Me 2 N-BAVAH, Drug Metabolism and Disposition , 2005.

• G. Elaut, T. Vanhaecke, S. Hebbrecht, D. Tourwé and V. Rogiers. Toxicological screening of the histone deacetylase inhibitors Trichostatin A and 4-Me 2 N-BAVAH in primary rat hepatocyte cultures, Toxicological Sciences, 2005.

• G. Elaut, V. Rogiers and T. Vanhaecke. The pharmaceutical potential of histone deacetylase inhibitors, Current Pharmaceutical Design, 2005.

• M.R. Daha .Regulation of IL-6 production by human tubular epithelial cells and the renal epithelial cell line HK2. 2006. Manuscript in preparation.

• Characterization of primary rat proximal tubular cells by gene expression analysis, Weiland C., Ahr HJ., Vohr HW., Ellinger-Ziegelbauer H.

• Hepatocytes from human adult and embryonic stem cells: functional characterisation and comparison with human hepatocytes. Sabine Gerbal-Chaloin, Natalie Funakoshi, Cédric Duret, Astrid Herrero, Patrick Maurel and Martine Daujat-Chavanieu.

• Differentiation of CD105^-/CD26⁺ non parenchymal epithelial cells to hepatocyte-like cells: influence of cellular matrix. Duret C, Gerbal-Chaloin S, Ramos J, Fabre J.M, Jacquet E, Navarro F, Blanc P, Sa-Cunha A, Maurel P, Daujat-Chavanieu M..

• Hepatocytes from human adult and embryonic stem cells. Sabine Gerbal-Chaloin, Natalie Funakoshi, Cédric Duret, Astrid Herrero, Patrick Maurel and Martine Daujat-Chavanieu.. Review article, for Gastroenterol Clin Biol 2008.

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